What should be the optimum CRISPR/Cas9 virus titer to knock out a gene in primary lymphatic and/or endothelial cells?

Hi All, I want to knock a gene using CRISPR/Cas9 technology. Previously I have used lentivirus to knock down gene expression in primary lymphatic endothelial cells (LEC). In primary LEC, I used to...

20 August 2018 2,364 0 View