Thanks to anyone how would like to spare me attention first. I am working on treating primary mouse hepatocyte with drugs may cause dyslipidemia, hoping to find any positive results on their influences. I have a master question as, what kind of treating medium should I choose? Currently I am applying William E with CM 4000 supplement for both maintaining and treating, while I feel like that too much supplements may have unexpected influence on the outcomes. However, I also wonder that applying poor medium like William E only would strongly damage primary hepatocyte functioning, which may also cause unwished results. May anyone with experiences help me with this?

Besides, I also need a positive control to validate both the SREBP pathway upregulation and cholesterol increasing. Statins can upregulated the SREBPs and Hmgcr by blocking their activity, causing cholesterol lowering, which is not ideal for me. May anyone also have ideas about this can help?