How should I make a positive control of autophagy by NH4CL?Which concentration is good for nalm?

29 May 2022 0 10K Report

Hi everyone ,does any one know how can I make a positive control for autophagy pathway with NH4Cl? I’m working on nalm6 cell line

Badges
Science topic

More Elmira Zarei's questions See All

What articles do we have on the use of body language among politicians for policy conception?

need the latest articles on the powerful use of body language among politicians for policy conception and how politicians leverage body language to influence public opinion and gain support.

10 March 2024 5,036 2 View

In calculating the efficiency and production of solar water desalination along with the collector, should the area of the collector be included in it?

"Please help me answer this question."

25 January 2024 2,768 4 View

Which kind of acid is suitable to wash glass equipment?

I need to wash glass ampoules to remove organic residues and other chemical contaminant. Which kind of acid and which concentration of it is needed?

06 January 2024 4,266 3 View

Methanol & combination of Methanol with Water?

Hello to all Does anyone have reliable information about the chemical properties of methanol and the combination of methanol with water? With valid reference We need this information to select the...

25 December 2023 8,901 4 View

What is the difference between vector pET (26,21, a,,b,+.........?

What is the difference between vector pET (26,21, a,,b,+.........? What are the changes made in the PET vector (for different pET)?

06 December 2023 5,576 1 View

What is vosviewer thesaurus file correct lable?

Hi I have problem importing thesaurus file to vosviewer. the error is ".... is not valid column header". whatever the first line is, the error repeat as the same. should I make changes to file and...

13 October 2023 1,655 1 View

I am looking for an organic substance that can precipitate dissolved zinc and remain on its own?

Zinc replaces most of the compounds, and the solution contains significantly more zinc, the goal being to recover the zinc for reuse.

04 August 2023 3,334 0 View

Is it possible to use ozone as the input gas source of cold plasma?

I have seen in many studies, oxygen is used as the plasma gas source to produce ozone in the plasma chamber. But my first question is, are there any advantages if ozone is directly used as the...

03 August 2023 9,035 2 View

I did the MTT test for Colchicine. Unfortunately, colchicine interferes with the results of the test. How can I solve my problem?

Mtt assay

20 May 2023 709 2 View

PGA changes with depth?

In international standards (ASHTO, FHWA, etc.) and many reports of engineering companies, for deep underground structures (deeper than 30 meters), when PGA is transferred to the depth, a reduction...

19 May 2023 738 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View