In our lab we have extensive experience in freezing PBMCs, and we always cryopreserve them in FBS as this is how we obtain the highest viability. However, we are about to start working with Jurkat E6.1 cells, which are tumour T lymphocytes. The commercial establishment recommends freezing them in culture medium supplemented (with 10% FBS), and 10% DMSO. So, which are the best conditions to cryopreserve our cells in the best possible conditions?
Hello Asier Ureta Camiruaga
The appropriate freezing medium for Jurkat E6.1 cells would be culture medium supplemented with 10% FBS and with either 5% or 10% DMSO.
Attached below are articles which may help you to improve upon the cryopreservation protocol to obtain good viability.
Article Proline pre-conditioning of Jurkat cells improves recovery a...
Article Physical events occurring during the cryopreservation of imm...
Best,
Hi, I am currently working on cryopreserving PBMCs from cord blood and adult blood, using cryostor, however, my cell viability have been very low after thawing, would you be able to share any tips you have for storing of the PBMCs. Thank you.
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