Asier Ureta Camiruaga

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Questions related from Asier Ureta Camiruaga

Why are my LN-18 glioblastoma cells starting to die after 5 days in culture?

I am growing LN-18 glioblastoma cells following ATCC's reccommendations (5% FBS, 1% p/s). I seeded 500.000 cells in a T75 flask, and cells seemed to grow without any problem. However after several...

17 March 2025 3,626 0 View

How can I measure proliferation of adherent cell lines using CFSE?

I am trying to measure cell proliferation in adherent cell lines using CFSE, but the peaks I observe are not well distinguishable and I usually observe only one peak. Could anyone who has...

14 March 2025 4,553 0 View

How should I cryopreserve my Jurkat E6.1 cells to obtain the highest viability?

In our lab we have extensive experience in freezing PBMCs, and we always cryopreserve them in FBS as this is how we obtain the highest viability. However, we are about to start working with Jurkat...

07 March 2025 748 2 View