What is the best strategy to extract total RNA (Trizol) from crab gills?

Dear all I have tried 1ml Trizol to extract total RNA from crabs gills. The weight of most of my samples is + -0,03 g. We have applied this protocol for hepatopancreas samples and the results...

03 June 2019 1,447 8 View

Do you know what this small lab item is?

Hi, I found these little circle caps/membranes(?) in my lab, they are stored in an odourless liquid that is not water (I tried mixing some in and could see the fluids mixing). I work in a...

19 February 2019 7,404 7 View

How to enrich for CD34+ population from a cell line (without cell sorting)?

I wanted to know if enrichment kits can also be used for cell lines or if they can only be used for buffy coat and peripheral blood samples? Thank you!

28 January 2019 7,351 3 View

What concentrations to use for receptor (protein) in fluorescence polarization when KD is not known?

I am trying to determine the binding between a protein and cofactor, however the KD value cannot be found online/published. Would the concentrations of my receptor I choose for my assay be more of...

10 May 2018 6,029 5 View

How to extract yeast DNA to meet demands of PacBIo sequencing?

14 July 2016 6,869 3 View

Does anyone know which software can be used for analysing nutrients in exotic foods?

29 January 2015 3,533 7 View

What is the role of citrate phosphate in substrate for ELISA?

13 August 2014 3,649 1 View

K562 cells marker expression

Does anyone have experience with the markers expressed on K562 cells? I have checked DSMZ and when I stain my cells with the markers stated that K562 should be positive for and run the profile on...

01 January 1970 563 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View