What is the best strategy to extract total RNA (Trizol) from crab gills?

Dear all I have tried 1ml Trizol to extract total RNA from crabs gills. The weight of most of my samples is + -0,03 g. We have applied this protocol for hepatopancreas samples and the results...

03 June 2019 1,447 8 View

How to enrich for CD34+ population from a cell line (without cell sorting)?

I wanted to know if enrichment kits can also be used for cell lines or if they can only be used for buffy coat and peripheral blood samples? Thank you!

28 January 2019 7,351 3 View

What concentrations to use for receptor (protein) in fluorescence polarization when KD is not known?

I am trying to determine the binding between a protein and cofactor, however the KD value cannot be found online/published. Would the concentrations of my receptor I choose for my assay be more of...

10 May 2018 6,029 5 View

How should healthy HAC15 cells look?

I'm a quite new to growing cells and have only worked with HAC15-cell line and H295R-cell line. The HAC15-cells look very different from H295R, and I'm starting to wonder if they are healthy or...

24 September 2017 1,038 1 View

How to extract yeast DNA to meet demands of PacBIo sequencing?

14 July 2016 6,869 3 View

Does anyone know which software can be used for analysing nutrients in exotic foods?

29 January 2015 3,533 7 View

What is the role of citrate phosphate in substrate for ELISA?

13 August 2014 3,649 1 View

K562 cells marker expression

Does anyone have experience with the markers expressed on K562 cells? I have checked DSMZ and when I stain my cells with the markers stated that K562 should be positive for and run the profile on...

01 January 1970 563 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View