Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

Selecting particular columns from large datasets (csv) using R Studio?

So I have large number of csv files (100+) in a directory with same structure and column names. The data is in time series and there are approx 30 columns/file. However i only want to aggregate...

08 February 2021 245 5 View

In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it OK??

In my CFD simulation analysis, I found high turbulence intensity with low indoor air velocity in a naturally ventilated room. Is it ok??

25 January 2021 5,336 10 View

How can solve the second order linear homogeneous partial differential equation with variable coefficients?

can someone help me with reduced the partial differential equation du/dt = - f(x) d^2 u/ dx^2 - g(x) du/dx --- (1) to become: du/dt = - p(x) d^2 u/ dx^2...

21 January 2021 3,296 1 View

IHC-paraffin experiment: how do you approach shopping for an antibody?

Hello everyone! I am shopping for an arginase 1 antibody to use for IHC-paraffin experiments with rat tissue. So far what I have understood is that if an antibody says it is reactive with rat,...

16 January 2021 794 3 View

Selection of research topic for phd in management any one suggest me what should be topic of research for phd thesis ?

i want guidance from seniors that what is field of research in management, marketing field which is important in present era please suggest

01 January 2021 3,901 4 View

Any recommendation to calculate time to dominant design emergence?

Any recommendation to calculate time to dominant design emergence?

01 January 2021 3,809 5 View

Are drones following the e-Auto industry technological trajectory or are they following its own?

* Are drones following the e-Auto industry technological trajectory or it is following its own? * Will drones diffuse faster than electrical vehicles? * Will it depend on the application?, What...

29 December 2020 7,379 3 View

Parameters for Social Media Viability?

I am working on social media viability for political campaigns, so your suggestions are highly appreciated what parameters should use to analyze Social media viability for political campaigns

27 December 2020 9,010 2 View

How to explain insignificant independent variable which is significant with interacting (Moderation) variable?

10 December 2020 3,037 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View