As some evidences of dual role of GM-CSF as a pro-Inflammatory and a regulatory of cytokine, How relevant is the cellular, tissue, or organ source of GM-CSF production, or the cytokine milieu "of the surroundings"?
Dear colleagues Can I apply biofloc system in earthen ponds?
17 May 2024 2,685 0 View
Hello everyone! I am working on my undergraduate thesis. After I used the DNeasy® PowerSoil® Pro Kit to extract DNA from a coral mucus sample I use 2 microliters of DNA to measure the output's...
15 December 2023 4,751 1 View
Dielectric permitivity spectrogram interpertation and cole cole plot especially for biological material
09 March 2023 5,691 0 View
I am attempting to detect a number of proteins expressed by a bacterium during infection in a mouse model. However, the presence of mouse proteins in the serum and tissues is hindering the results...
02 February 2023 2,629 1 View
Has the development of technology led the human mind, especially the educated, to a lack of absorption and difficulty in learning despite the availability of technological means, unlike the...
27 October 2022 7,633 14 View
Hello, I received this SPR sensogram for small molecule binding affinity test on a protein. Would you please explain to me what are those dotted lined next to solid line? Thanks
19 May 2022 1,258 3 View
I need to dissolve it in water for the preparation of SEM-TB media. I prepared 1M stock solution but it is cloudy not clear. I can not use it in the media preparation as it has to be clean.So what...
01 December 2021 3,545 5 View
Is there anyone who can give me some studies and statistics for brain drain from North Africa to Europe and North America?
01 November 2021 7,256 3 View
I have a drug that comes in 10mg, how much would I add to obtain a final stock concentration of 100mM? The MW is 579.9and the drug is soluble in H2O at 52mg/ml. When I did the calculation I...
18 October 2021 2,745 3 View
International Conference of Harmonization (ICH) guideline M7 recommends using two complementary QSAR models, expert-based and statistical-based models, for Genotoxicity prediction. I'd be thankful...
12 August 2021 4,357 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View
I am planning to prepare a decellularized porcine tissue derived biomaterial. It would be very helpful if I get to know the composition of the solution which is ideal to bring the tissue sample...
31 July 2024 5,645 2 View
I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....
31 July 2024 3,665 3 View
I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...
29 July 2024 3,030 2 View
My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...
29 July 2024 2,682 6 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
This is a slice of intestinal tissue from the Chinese mitten crab (intestine in the upper right corner). I injected WSSV into this horseshoe crab. Now I want to know what these red spots next to...
18 July 2024 5,602 0 View