I am a PhD student at Tufts University and I have been asked to gather some feedback from the scientific community for a product development project.
Based on work being done in my lab we have seen cell-dish interactions having negative effects on the tissue culture, both limiting the desired cell-cell interactions as well as causing encapsulation in our insect cultures.
How often does this problem come up for those who regularly do cell culture?
Are treated plates sufficient in treating this problem?
If a product was offered that could further reduce cell-plate interaction would you be interested in trying it?
Based on what you described, hard to say anything. I could give you a few suggestions. Forgive me if I am wrong in guessing what would be happening in your hands in the lab. All my suggestions come from my experiences by doing experiments, teaching and supervising undergrads, grads and post-doc, and even very senior of colleagues.
First possibility is contaminated cells with bacteria or mycoplasma or even viruses. Cells tend to detach, making varying sizes of cell fragments and cell death. Some cells detached, but survived may make fluid-containing small balls of encapsulated clumps, in particular, in endothelial or epithelial cells (i.e. human breast cancer MCF7 cells). Just purchase the insect cell line and test with your current cells together, of course, and run a few commercially well-known electrically charged dish products together. Depending on the observation, you could tell whether your coating is properly done in terms of time and dosage of the coating the dish. Often high density of cells causes a similar phenomenon, specially in case of high-secreting ECM cells.
Secondly, check the medium by replacing with freshly-made one.
Lastly, you may find problem, if any, by testing other cell culture (well -known non-insect cells available surrounding you) with the product dish.
Unfortunately, I am retiring soon to be involved testing the product.
You will find the way by checking the above. Good luck to your homework.
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