Hi every one,
I am extracting enzymatic extract from plant (50 mM Sodium phosphate buffer (pH 7.8), 0.1 mM EDTA, 0.1 % (v/v) Triton; 1 mM PMSF). I mesure the protein content and enzymatic activity fom this extract.
So I want and I need to know if the enzymes extract can be conserved for further enzymatic activity measurement (SOD, CAT, APX, ...).
I really need your help, thank you very much
It is quite impossible to answer such a general question. Conservation will depend on a) the enzyme; b) the extract; c) the conditions of conservation : temperature, duration, addition of preservants (cryopreservants such as glycerol if freezing, protease inhibitors, antioxidants etc. My advice would be to check for yourself by performing assays on the same extract at various time intervals under defined conditions of conservation.
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