Can i use DTT during a Zn affinity chromatography?

I need to purify His6 tag containing protein and im using a Histrap that is been charged with Zn2+. Can i add DTT to my lysis buffer or the column will not tolerate it when i insert the lysate to...

12 October 2020 5,910 4 View

How can I solve a checkpoint file problem in Gromacs 2018.4?

Hello everyone, I am running a simulation for 500 ns but after 200 ns I get the following error: The input requested 100000000 steps, however the checkpoint file has already reached step...

16 January 2020 454 7 View

How to model Constant-Current Constant-Voltage Charging of Lithium-Ion Battery in Comsol?

I am trying to implement a constant-current constant-voltage charging strategy using the COMSOL standard lithium-ion battery model that comes with the chemical engineering module. The idea is to...

02 December 2019 9,563 3 View

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts)?

I am trying to figure out how quickly oxygen goes back into degassed E3 medium (essentially water with some salts). Degassing takes place with 5 cycles of 1 minute degassing in vacuum and 1 minute...

18 November 2019 5,423 2 View

SEB or SEE for Jurkat and primary human/mice CD4+ T cell activation?

25 July 2017 338 7 View

How to check the observability of a LPV system?

Consider the discrete system x' = A(t)x + B(t)u y = C(t)x' + D(t)u The matrices A, B, C, D contain time-varying parameters (calculated by a second model) which are given in the initial state, but...

19 May 2017 2,813 1 View

How to detect low amounts of mRNA by qPCR?

I want to detect early expression of IL2 by qRT-PCR. I was not successful using random hexameric primers at the RT step and SYBR for detection. what can I do to improve my sensitivity? What...

01 February 2017 7,322 4 View

How do I calculate sensitivity of android acccelerometers?

Hello, I would like to make a sensitivity experiment on android built-in accelerometers (tri-axonal) an make a statistical analysis (correlation etc) in Matlab. My questions are: 1..What would be...

08 November 2016 9,127 1 View

Any suggestions for efficient Raji cell transfection?

Hi everyone. Has anyone worked with Raji cell transfection? I recently used AMAXA technology to transfect Raji cells with a CRISPR construct that resulted in a very poor efficiency (~1%). When I...

17 May 2016 5,476 4 View

Could Raman spectroscopy reveal the presence of Pt and Ru nanoparticles dispersed on a carbon powder?

09 November 2015 8,352 5 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View