How much of EDTA-free protease inhibitor should be used when extracting protein?

01 January 2014 1 10K Report

I am going to extract protein from BL21 but I do not know how much of EDTA-free protease inhibitor (tablet from Roche diagnostic) should I use.

Hadi H Mohammad

Hi Paul

Thanks for your reply. please can you clarify what do you mean by 50 ml, do you mean 50 ml of protein or 50 ml of water ?

Regards

Hadi

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