Anyone know a suicide plasmid that can be used to delete a gene in E.coli strain?
I need to use a suicide plasmid to delete a gene in E.coli strain. I used one for gene deletion in Pseudomonas but that was an E. coli plasmid origin which can replicate normally in E.coli.
If there is one, which strain I have to construct the plasmid, for example for the deletion in Pseudomonas, I constructed the plasmid and propagated in DH5α, then I used S17-I to transconjugate it into Pseudomonas.
Your help and suggestion are highly appreciated
Armin Resch
see: Hamilton et al. 1989 (PMC210259) Link et al. 1997 (PMC179534) Datsenko & Wanner 2000 (PMC18686)
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