Anyone know a suicide plasmid that can be used to delete a gene in E.coli strain?

12 December 2017 1 228 Report

I need to use a suicide plasmid to delete a gene in E.coli strain. I used one for gene deletion in Pseudomonas but that was an E. coli plasmid origin which can replicate normally in E.coli.

If there is one, which strain I have to construct the plasmid, for example for the deletion in Pseudomonas, I constructed the plasmid and propagated in DH5α, then I used S17-I to transconjugate it into Pseudomonas.

Your help and suggestion are highly appreciated

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