Hi Everyone, We are searching the method about the lowest dosage of DMSO as a non-toxic dosage for direct injection cryopreserving cell in animal/human body which keep the cell quality?
#cryopresevation #live_cell
The lowest non-toxic dosage of DMSO (dimethyl sulfoxide) for direct injection into an animal or human body can vary depending on factors such as the specific application, the size and condition of the animal or human, and the concentration of the DMSO solution. DMSO is known to have a wide range of potential effects, and its safe and effective use requires careful consideration of these factors.
It’s important to consult with a qualified medical or veterinary professional to determine the appropriate dosage and administration for your specific situation. DMSO should only be used under the guidance of a healthcare professional, as improper use can lead to adverse effects. If you are considering using DMSO for a specific application, it’s best to seek advice from a healthcare professional who is knowledgeable about its potential benefits and risks.
DMSO (dimethyl sulfoxide) is a widely used solvent and penetration enhancer, and it has also been studied for its potential therapeutic properties. When considering the dosage of DMSO for direct injection, it’s important to take into account factors such as the specific intended use, the individual’s weight and health status, and the concentration of the DMSO solution.
For research or medical purposes, the dosage and administration of DMSO are typically carefully controlled and guided by established protocols. When used in animal or human studies, the dosage of DMSO is often determined through preclinical experiments to assess its safety and efficacy.
For direct injection, the dosage of DMSO should be determined by a healthcare professional or a veterinary expert based on the specific needs of the patient or animal. DMSO can have potential side effects and risks, and using an appropriate, non-toxic dosage is crucial to ensure safety and minimize adverse effects.
Given the complexity and potential risks associated with DMSO use, it’s important to seek guidance from a qualified professional who can assess the individual circumstances and provide personalized recommendations for the safe and effective administration of DMSO.
( AI answer )
Dear Dr. Maryam Hazeri
You may want to refer to the article attached below.
Article Tolerable Levels of Nonclinical Vehicles and Formulations Us...
Please see table 36 for information on DMSO.
Best.
Malcolm Nobre and Khashayar Danesh narooei Thank you so much for sharing information but I need the lowest dosage of DMSO as a non-toxic dosage or any substitute solutions which keep the cell quality and we can use for direct injection cryopreserving cell in animal/human body specially after transferring to another city
Dear Maryam, DMSO is a good opportunity for cryopreservation of cell products. In the publication referenced by Malcolm a single intravenous dose of 1.65 g/kg (30% DMSO) was tolerated in female CD-1 mice. The density of DMSO is 1.1 g/mL, the tolerated dose would be 1.5 mL/kg and for a mouse of 20 gram body weight these are 30 µL DMSO. The i.v. injection volume of your cell product is 5 mL/kg, e.g. the mouse receives 100 µL. Cell products are often cryopreserved with 5-10% DMSO, corresponding to 5-10 µL DMSO when 100 µL cell product suspension are applied. Based on these estimations I would assume that a single cell product dose containing 10 µL DMSO should be well tolerated.
Regarding a potential freeze-thaw induced cell damage I would recommend to assess the viability of the product at least 24 hours later. Depending on the determined viability you should adjust the cell dose in comparison to unfrozen cells (67% viability loss requires a 3-fold higher dose!).
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