Crystal Semaan

2 Questions 1 Answers 0 Followers

Questions related from Crystal Semaan

How much glucose to add to LB media to prevent leaky expression?

I am already using BL21(DE3)pLysS cells but I am still getting leaky expression in pre-expression samples before IPTG is added. LB broth is tryptone, salt and yeast. Thanks :)

29 May 2024 2,675 2 View

MCherry tag being photobleached during expression?

I am expressing an mCherry tagged protein. Yield of total protein is good but the fluorescence intensity is very low (ie. Protein assays and gels show a lot of protein but this does not correspond...

29 May 2024 4,792 1 View