How much effective is primary PCR product dilution?

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No title

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Sven Reister

Hi,

in principle Nested PCR is very effective. From this perspective and based on literature you can even dilute higher and should suceed. Therefore the higher dilution should be better as this will also reduce the amount of all other components form the first amplification like Primers etc

Best Sven

Md. Mahiuddin Zahangir

Hi Mehwish Nawaz

Usually i use 10X dilution for my work. Higher dilution is also possible to get results.