How much effective is primary PCR product dilution?

I have to amplify HVR-1 of Plasmodium falciparum ...I diluted primary PCR products 5X and 10X for nested PCR... and I got results in both..which one is more acceptable or effective...

01 February 2019 8,837 4 View

Can anyone help me. Need assistance in PCR I couldn't amplify HVR1 of AMA1 gene in P. falciparum i am following conditions given in Article but still?

I have to amplify HVR1 of AMA1 in Plasmodium falciparum isolates...Annealing temperature given in articles is 55 o C and i am also following all the conditions but still i didn't get positive...

01 February 2019 1,774 4 View

How to Purify Nucleic acids by using Affinity chromatography?

Separation of Bio-molecules by affinity chromatography Most specifically i am in search of DNA and RNA purification by using affinity chromatography.... As DNA affinity chromatography and RNA...

11 December 2018 3,512 4 View

How to interpret Haplotype Network?

I have drawn Haplotype Network using Network 5.0. What does the median vector tell us?

10 November 2018 218 0 View

Not getting PCR results

I need your valuable suggestion in my Research I have to amplify HVR-1 of malarial patients infected with Plasmodium falciparum For which i have to perform two PCR Primary and Nested PCR The...

01 January 1970 6,515 34 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Can water be extracted from Olivine?

I have always been interested in geology and wondered about how unique structures of materials behave in different circumstances. I stumbled upon Olivine, the magnesium iron silicate, and heard...

28 February 2021 7,330 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View