Mehwish Nawaz

6 Questions 29 Answers 0 Followers

Questions related from Mehwish Nawaz

How much effective is primary PCR product dilution?

I have to amplify HVR-1 of Plasmodium falciparum ...I diluted primary PCR products 5X and 10X for nested PCR... and I got results in both..which one is more acceptable or effective...

02 February 2019 8,915 4 View

Can anyone help me. Need assistance in PCR I couldn't amplify HVR1 of AMA1 gene in P. falciparum i am following conditions given in Article but still?

I have to amplify HVR1 of AMA1 in Plasmodium falciparum isolates...Annealing temperature given in articles is 55 o C and i am also following all the conditions but still i didn't get positive...

02 February 2019 1,842 4 View

May I use Bligh and dyer method for lipids extraction from egg yolk?

I have to extract Lipids from Egg yolk.... May I follow the Bligh and dyer protocol...

12 December 2018 2,118 6 View

How to Purify Nucleic acids by using Affinity chromatography?

Separation of Bio-molecules by affinity chromatography Most specifically i am in search of DNA and RNA purification by using affinity chromatography.... As DNA affinity chromatography and RNA...

12 December 2018 3,595 4 View

How to interpret Haplotype Network?

I have drawn Haplotype Network using Network 5.0. What does the median vector tell us?

11 November 2018 329 0 View

Not getting PCR results

I need your valuable suggestion in my Research I have to amplify HVR-1 of malarial patients infected with Plasmodium falciparum For which i have to perform two PCR Primary and Nested PCR The...

01 January 1970 6,575 34 View