I have drawn Haplotype Network using Network 5.0. What does the median vector tell us?
I have to amplify HVR-1 of Plasmodium falciparum ...I diluted primary PCR products 5X and 10X for nested PCR... and I got results in both..which one is more acceptable or effective...
01 February 2019 8,837 4 View
I have to amplify HVR1 of AMA1 in Plasmodium falciparum isolates...Annealing temperature given in articles is 55 o C and i am also following all the conditions but still i didn't get positive...
01 February 2019 1,774 4 View
Separation of Bio-molecules by affinity chromatography Most specifically i am in search of DNA and RNA purification by using affinity chromatography.... As DNA affinity chromatography and RNA...
11 December 2018 3,512 4 View
I have to extract Lipids from Egg yolk.... May I follow the Bligh and dyer protocol...
11 December 2018 2,011 6 View
I need your valuable suggestion in my Research I have to amplify HVR-1 of malarial patients infected with Plasmodium falciparum For which i have to perform two PCR Primary and Nested PCR The...
01 January 1970 6,515 34 View
Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...
03 March 2021 401 4 View
I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...
02 March 2021 4,362 1 View
Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
02 March 2021 5,785 3 View
Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...
28 February 2021 4,868 2 View
Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...
27 February 2021 1,965 1 View
I'm currently working on a piece which asks to conduct an ANOVA - looking at the effect of sporting group (2 levels), and exercise trials (two trials with six levels). I've computed this all...
27 February 2021 9,626 4 View
I have obtained Partial Eta Squared Numbers through Two-Way Mixed Anova Analysis. My results for different constructs are: .105, .135, .038, .068, .061 respectively. I wanted to know, whether...
24 February 2021 7,122 1 View
Dear colleages, Eta squared is dominantly used in reflecting the explaining power the same manner as a squared partial correlation coefficient (R2p ) from multiple linear regression: as the...
22 February 2021 7,801 3 View
Hello, i work with a spectrometer which only render the fluorescence emission spectrum. Until now i just found how to interpret an exitation- and emission spectrum together to generate deeper...
20 February 2021 1,291 3 View
For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...
20 February 2021 4,332 4 View