Hi,
I would like to know how much DNA you generally get from soil and/or sediment. I did some extractions but I am not sure if what I have got can be considered a good amount or not.
I use kits (from qiagen and mpbio). The DNA concentration of the elution usually ranges between 15 ng/ul up to 80 ng/ul.
I've also got very bad 260/230 values from my samples, which should indicate the presence of contaminants.
Can someone share his/hers knowledge, please?
Thanks
Dear Valentina!
You look at this article, please:
Article Simultaneous Recovery of RNA and DNA from Soils and Sediments
With the optimized protocol, intact RNA and high-molecular-weight DNA were simultaneously recovered from 19 soil and stream sediment samples of diverse composition. The RNA yield from these samples ranged from 1.4 to 56 μg g of soil−1 dry weight), whereas the DNA yield ranged from 23 to 435 μg g−1.
I usually get more than 200 ng from my samples. What is your sample type?
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