How much concentration of the PI should be used for staining of the cells in 35mm petridish with 14mm microwell?

21 May 2024 0 6K Report

Does the higher concentration of the PI stains the nucleus of the live cells as well? In order to confirm the membrane damage in one region I used the PI dye with the Hoechst dye. I got positive result for the PI in the live cells as well. Does PI passes through the membrane via transporters or cells with leaky membranes?

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Science method

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How to do calculation of Louvain methods calculation for excel sheet and and is there any software available?

The Louvain method – named after the University of Louvain where Blondel et al. developed the algorithm – finds communities by optimizing modularity locally for every node’s neighborhood, then...

23 July 2024 6,659 0 View

What is information diffusion in the social network?How a message got viral in social network?

Please give answer. Also explain mathematical equations behind this.

22 June 2024 6,869 2 View

How to determine the melting or boling point of any chemical which is in the poweder form?

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24 May 2024 2,932 4 View

How to get dataset in social network analysis for decision making?

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03 May 2024 4,367 1 View

If cri is fixed in coke , can we reduce csr?

As per the demand of blast furnace, cri should be 23 to 24, is it possible to get csr between 63 to 64?

24 March 2024 5,183 1 View

How to do Prediction of unsupervised dataset using machine learning?

Please give valuable information.

02 February 2024 6,177 10 View

Elastomer exposed through the sputtering target?

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18 December 2023 1,209 1 View

How do I increase the overall and kappa accuracy of LULC image ?

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06 December 2023 2,321 2 View

What types of dataset are required to study trust analysis in social network?

Please give some details.

05 December 2023 282 0 View

I have five subgroups of Facial Index (in deffreent range) and five subgroups of one personality (in range), which test I should use?

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25 September 2023 800 2 View

How to increase citation in Research Gate?

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04 August 2024 3,368 4 View

Does matter flow through ecosystems and energy is recycled and how does the flow of matter and energy compare in the living world?

03 August 2024 607 2 View

Interested in a SCOPUS collaboration?

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How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?

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How Do Project Data Analytics and AI Advance Quality 4.0 in Construction Project Management?

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What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

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H-index issues?

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Is it necessary to attach fluorophore at 3' end in MIC RT PCR machine for Dual hybridization Probe designed especially for genotyping assay?

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Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM images?

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25 July 2024 5,482 2 View

Would you be willing to take a look at interview questions for a research study?

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