Can anyone explains about this question related to this manuscript?

Dear All, I need your help asap. I have gone through this paper and want to understand in-depth and resolve this question. Please have a look at it and give me a possible understanding. I have to...

12 January 2020 4,923 1 View

PLGA np size range from 80-220nm effect on cells?

We prepared PLGA np of different sizes ranging from 80, 100, 120,...., 200, 220nm. Do you think it has an effect on cell lines. Cellular uptake behaviour internalization and MTT assay.??

25 March 2018 2,398 3 View

How to perform the Recovery in different strength of tables/Capsules by HPLC during assay and related substance test?

How to perform the Recovery in different strength of tables/Capsules by HPLC during assay and related substance test?

20 November 2017 127 3 View

How to normalise fluorescent images taken at different exposure times?

I did a few experiments with fluorescent microscopy. The goal of the study is to look at diffusion of 100 nm rhodamine labelled fluorescent particles with time. As images had to be captured at...

20 May 2014 3,535 4 View

What electrolyte and parameters should we use to electropolish tungsten based W-Ti and W-Ta alloys for EBSD characterization?

The sample surface is turning grayish-black with 2% NaOH electrolyte as suggested in literature. Please suggest a suitable electrolyte with parameters to prepare tungsten based W-Ti and W-Ta...

03 January 2014 483 6 View

Blast analysis of RC,UHPC slabs using material model *MAT_72R3 in LS-DYNA?

20 February 2021 5,984 3 View

Good day sir/ma. Please, where can I have access to equipment for conducting XRF, XRD and SEM analysis?

I'm using blast furnace slag for stabilizing subgrade soil.

17 February 2021 7,333 3 View

Is nr blast important?

Hello, I have designed a specific primer for my Gene, when I blast this primer, it just finds the mRNA of the gene, but in nr blast, I have found some CDSs that are not relevant to my Gene. Do...

05 February 2021 5,842 3 View

Abaqus - plate is going up under gravity+blast loads?

Hi, I have modeled concrete plate with reinforcement supported on 2 concrete block. between concrete block and the plate I have added regular contact. I have created 2 steps: 1) only gravity load...

30 January 2021 2,772 1 View

Is there any alternative to BUSCO for searching orthologues within genomes available?

I have a need to search for single-copy conservative orthologs within several plant genomes and used BUSCO tool for that. However, I found that the results produced by different versions on the...

29 January 2021 7,764 6 View

How to check "no blast hit" sequences of NGS data output?

My environmental sample was analyzed for fungi by both DGGE (18S) and NGS (ITS2), resulted in some different names (in the top 5 of strong bands in DGGE, or most abundant sequences in NGS) between...

21 January 2021 5,310 3 View

Best way to optimize species-specific primers? Can I use a temp gradient to test a Ta where I will get target (and no non-target) amplification?

Hello, I am testing different species-specific primer sets. I have two targets (two species, but same gene target). I am trying to select the best primers -- one that will amplify the target...

18 January 2021 1,981 6 View

Can BLAST/ search by using known Domain in Transcriptome/Genome data for a gene family?

Dear Researchers, I have Transcriptome/Genome data and want to list all the member of a KCS/CER gene family whose Domain AP2/ERF, FAE1_CUT1, according to the Arabidopsis database, so is there...

15 January 2021 2,293 3 View

How can I build a local KEGG db?

I want the info (gene name, metabolic pathway, and organism of origin) of some key identified metatranscriptome genes obtained from MG Rast (I have the sequences). The KEGG ftp site seems to...

12 January 2021 9,985 1 View

Why do I keep obtaining the same secondary PCR band along with the specific product band?

Hello everyone! I need to amplify a specific DNA fragment in order to do Sanger sequencing for the detection of a point mutation in human patients. The fragment is in an exon of my gene of...

25 December 2020 1,805 3 View