How MOG induced EAE in mice?

01 January 1970 0 9K Report

how PHA/MOG35-55 treatment induced changes in cytokines in the spleen and how this is related to SMC proliferation and antioxidant reduction in blood serum ?

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Science method

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Please introduce AI website for paper review like Chat GPT?

01 March 2024 3,173 5 View

Problem with single step culture kitazato?

Hello I am from an infertility center in Iran. We have been using embryo culture medium. After a few days and the formation of embryos, the color of the embryos gradually turns black. While this...

26 November 2023 4,590 0 View

Kinematic transformation of CA spindle-tilting machine?

Hi dear colleagues, I failed to find the transformation matrix from the 3-axis (workpiece coordinate) to the 5-axis XYZAC (machine coordinate) table-tilting setup. Does anyone know any source?

07 November 2023 6,284 0 View

Clarification on "Space of Economy" vs "Place of Economy" - Seeking Insights from Scientific Articles?

Dear Researchers, I hope this message finds you well. I am currently delving into the concepts of "space of economy" and "place of economy," particularly in the context of economic geography,...

19 August 2023 3,196 2 View

What inflammatory biomarkers should be measured in individuals with non-alcoholic fatty liver disease(NAFLD)?

There are many biomarkers such as TNf-Alpha, CRP, and IL-1, but I'm looking for some novel biomarkers to measure.

21 March 2023 5,346 2 View

Transfer happens in only one cassettes of Bio-Rad Trans-Blot system. Any idea?

For years we are using Bio-Rad Trans-Blot Cell for Western Blot Transfer. It was working fine until recently we have a trouble in the transfer on the second cassette. The transfer in the first...

22 September 2022 2,244 1 View

How can I disperse C3N4 nanosheets?

After sonicating, my solution precipitated, pleased help me.

13 August 2022 7,313 0 View

How do we calculate the sublimation energy in f titanium in vacuum?

I was trying to calculate the sublimation energy but of Ti in 10^-8 torr. I understand that sublimation energy equals: E(delta t in solid)+E(solid to liquid)+E(delta t in liquid)+E(liquid to...

20 November 2021 1,169 3 View

What happens if the autocorrelation is considered incorrect in volatility estimation?

In time series modeling and volatility estimation it is necessary, first remove autocorrelation of time series and after that estimate the volatility model (like GARCH). the autocorrelation...

02 October 2021 2,488 4 View

Any company doing single-Cell total RNA Sequencing and Its Combination with Protein and DNA Analyses?

I would like to do single cell sequencing of DNA and total RNA in the same cell . My interests on noncoding RNAs. Do you know any cmany doing that? In the publications I read about SIDR-seq. But I...

01 October 2021 4,458 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

Cell proliferation assays suggestions?

What cell proliferation assay do you suggest? cyQUANT from thermo Fischer has good reviews, a lot of good articles for the kit from Abcam. and Promega kits look easy and straight forward.

21 July 2024 1,554 2 View

How could I determine antioxidant activity of Capsicum annuum fruits?

I need a detailed protocol of any suitable method for ex., DPPH to determine antioxidant activity.

28 June 2024 3,855 2 View

To what degree does the immunohistochemical (IHC) evaluation of Ki-67 hold diagnostic value beyond neoplastic pathologies?

In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...

25 June 2024 7,825 2 View

Can I dissolve DPPH in THF for antioxidant activity Assay ?

My compounds are soluble In THF, so I woould like to know If for antioxidant activity I can prepare DPPH in THF. Before, I did a test in wich I had DPPH in ethanol, but when I Add my sample in...

17 June 2024 4,183 3 View

Depletion experiments fo these three antioxidants in Atlantic Salmon?

Are there depletion experiments for these three antioxidants in Atlantic Salmon?

16 June 2024 1,915 1 View

Problem with nucleofection RNP delivery to murine primary T cells?

I have been using the Lonza nucleofector for RNP delivery to OT-I T cells without any issues for the past six months. However, recently, my nucleofected OT-I T cells have not been proliferating in...

13 June 2024 2,279 0 View

Why does the antioxidant activity increase as the number of OH groups on the phenolic ring increases?

and Why do double bonds reduce it ?

13 June 2024 4,836 4 View