Can RNApolymerase bind to RNA?

Given that the core enzyme tends to DNA in the cell, because of the electrostatic force. Why is there no such tendency between RNApolymerase 2 and RNA?

14 December 2020 2,708 5 View

How can I insert an assembeled system, meshed by SOLIDWORKS, to ANSYS?

I already did it before but this time as the model is complicated I have faced with tis error: The surface mesh is intersecting or close to intersecting,making it difficult to create a volume...

05 September 2020 9,558 1 View

Is there a way to calculate energies for all residues from a pdb?

CHARMM GUI online does a protein-protein interaction where you can measure the interaction energy of one chain with all the others. Is there a way to do this for each residue? Basically I want to...

29 July 2020 8,851 3 View

Role of intensity in UV-Vis Spectroscopy?

I have two suspensions of Gold Nanorods in Deionized Water synthesized by the same experimental route. Each of the GNR solutions shows the same peak but with different intensities. Is this related...

14 July 2020 9,680 0 View

What are the methods used to amplify the voltage received from two electrodes used in a micro channel to count micro and nano particles?

i'm recently on counting of micro & nano particle by methods like:impedance spectroscopy And I want to get a stronger voltage from my electrodes Who can give me a solution in this matter?

30 June 2020 6,963 8 View

Why would we see generated cracks during heat treatment of the additive manufactured Inconel 625 ?

18 June 2020 2,618 5 View

I want to compare the difference of immune system in patient with mild and sever symptom.who should i define the case and control group?

Is it enough to compare people who needed to be hospitalized with people who did not? , or should the age and duration of the illness be considered?

26 May 2020 2,829 2 View

how can i removed the tackiness of polyborosiloxane (poly borodimethylsiloxane)?

I have synthesized a PBS. this material have a tackiness after synthesized and stick the aluminum foil. how can i removed the tackiness of polyborosiloxane (poly borodimethylsiloxane)?

23 April 2020 2,161 3 View

Why the covid-virus infection is very rare in children compare to young and adult people?

As we know the immune system in both children and adult is impaired. The question is that why the covid-virus infection is very rare in children compare to young and adult people

03 April 2020 9,934 11 View

Is it methodologically true to combine effect sizes in a meta-analysis of clinical trial studies performed on children and adults together?

Recently, I have faced with a meta-analysis of clinical trials which included studies performed on adults and 4-8 years old children and combined the effect sizes of both population groups in the...

24 January 2020 7,571 3 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

How to accurately measure cytokines levels for my university research proposal?

Hello, i am writing a theoretical research proposal for one of my university assignments regarding Covid-19. The brief is pretty relaxed and i have chosen to conduct a study on how the...

11 February 2021 908 5 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

What M1 and M2 markers can I use for flow cytometry ?

Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...

26 January 2021 299 3 View

Can anyone help to find any research articles related to the below mentioned objective?

Astaxanthin used in surimi based products as colour enhancer or as an antioxidant! Regards & Thanks Asik Ikbal

25 January 2021 9,399 4 View

How to improve my BrdU immunostaining?

24 January 2021 414 3 View

How to lessen cell growth?

I am working on the 4T1 murine breast cancer cell line. from the first day I bought this cell line, the cell's growth rate is extremely high. I intend to make these cells resistant to a single...

24 January 2021 2,884 3 View