I want to know sample preparation and assay conditions to assess dimer:monomer NOS rather than total NOS
Divaker Choubey
To detect the dimer of any protein that are covalently linked, one needs to use sample buffer without any reducing agent. See details to detect dimer of NOS in link below.
Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3707674/
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