To model the BBB using transwell-membrane, I use bEnd.3 cells. The permeability of the monolayer is assessed by bypass of the fluorescent tracer Na-fluorescein through the monolayer.
Hello, I noticed a problem with my HPLC analysis and wanted to ask for help. During my analysis I've noticed repetitive problems with the baseline: it is dropping and then (usually) going back...
20 May 2024 6,501 3 View
Hello I am looking for information about osmophilie microorganisms and specifically their enzymes. If there are any industrially important enzymes from microorganisms living in environment where...
19 May 2024 136 1 View
I am preparing an experiment in which I would like to sample roots of sunflowers grown in pots (substrate is soil) in later stages of the plant development. Can anyone tell me, what is the best...
29 April 2024 8,775 1 View
Hi, Does my TaqMan probe recognize the gene only if the sequence has 100% coverage with the probe or will I also get signal if there is a SNP or even a few bp difference? And if a few bp are ok...
09 April 2024 6,856 3 View
I have tested enzyme activity (b-galactosidase) with cation ions in different buffer and the results differ dramatically. For example, while I used MES buffer calcium acts as an activator but when...
11 February 2024 3,135 2 View
Hello, Does anyone have any kind of experience with ordering cell lines from Accegen? I'm wondering if they are trustworthy and about the quality of provided cell lines?
26 November 2023 3,757 1 View
I think that mentoring programmes support in meeting publishers' expectations.
05 October 2023 2,583 1 View
I need to get information about the number of chromosomes or DNA content. I have seedlings from in vitro that were frozen at -20 degrees after liquidation of the culture. They are seedlings from...
28 September 2023 6,373 2 View
I need to detect phosphorylated IGF1R in cell lysate and brain lysate with Western Blot. Are there any life hacks for detection of such proteins?
05 September 2023 7,309 0 View
https://webmagazine.unitn.it/en/evento/sociologia/115480/esa-rn12-environment-and-society-summer-school
10 March 2023 3,806 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 639 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,891 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,834 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,891 4 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View