How many times can we use the DEAE cellulose column for the separation of compounds?

04 April 2014 0 5K Report

I already used the DEAE cellulose column once for separating compound, how many times we can reuse the column with proper washing?

I used 1 M NaCl as the elution solvents so, which solvent is better for washing the column for better use?

Badges
Science method

More Kusuma Venumadhav's questions See All

Can anybody explain how to calculate dose dependent IC50 values for the compound anti-oxidant activity?

See above

04 May 2014 4,154 9 View

How can we check the proliferation rate in the normal cell lines?

Is there any method to check the proliferation rate in the normal cell line apart from the Brdu FACS analysis, MTT or other cytotoxicity assays?

03 April 2014 6,640 11 View

Are there any standard polysaccharides with phenol rings?

I isolated polysaccharide from a plant and its giving strong fluorescence in UV light, but not giving any quenching with AlCl3 in UV absorption spectrum, I hope it contains a aromatic ring. Apart...

03 April 2014 6,531 3 View

What to expect from a sample I extracted? Polysaccharides or lignins?

I extracted a dried plant sample with hot water and added ethanol. I got PPT, then separated PPT and air dried it. Most of the literature says that it may be polysaccharides and it is soluble...

03 April 2014 5,859 9 View

How to fix emission and excitation wavelength in fluorescence spectroscopy?

I am working with three different plant species where I have extracted the plant material using four different solvents. How do I fix an excitation or emission wavelength for comparing the three...

02 March 2013 8,835 17 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View