Kusuma Venumadhav

6 Questions 7 Answers 0 Followers

Questions related from Kusuma Venumadhav

Can anybody explain how to calculate dose dependent IC50 values for the compound anti-oxidant activity?

See above

05 May 2014 4,108 9 View

Are there any standard polysaccharides with phenol rings?

I isolated polysaccharide from a plant and its giving strong fluorescence in UV light, but not giving any quenching with AlCl3 in UV absorption spectrum, I hope it contains a aromatic ring. Apart...

04 April 2014 6,477 3 View

How can we check the proliferation rate in the normal cell lines?

Is there any method to check the proliferation rate in the normal cell line apart from the Brdu FACS analysis, MTT or other cytotoxicity assays?

04 April 2014 6,613 11 View

What to expect from a sample I extracted? Polysaccharides or lignins?

I extracted a dried plant sample with hot water and added ethanol. I got PPT, then separated PPT and air dried it. Most of the literature says that it may be polysaccharides and it is soluble...

04 April 2014 5,825 9 View

How many times can we use the DEAE cellulose column for the separation of compounds?

I already used the DEAE cellulose column once for separating compound, how many times we can reuse the column with proper washing? I used 1 M NaCl as the elution solvents so, which solvent is...

04 April 2014 4,506 0 View

How to fix emission and excitation wavelength in fluorescence spectroscopy?

I am working with three different plant species where I have extracted the plant material using four different solvents. How do I fix an excitation or emission wavelength for comparing the three...

03 March 2013 8,731 17 View