What is dilution factor, how can we calculate it for our sample in drug release studies?

I want to check the drug release percentage. 2mg initial drug, 25mL buffer solution, 2 mL solution was taken out to check absorbance by UV and replaced with 2mL fresh buffer solution, What will be...

15 September 2020 5,099 1 View

What is the formula to calculate drug loading and drug release percentage?

I am working on drug loading to Lyotropic liquid crystalline gel to check sustained release (analysis by UV-visible spectroscopy).What is the formula to calculate drug loading and drug release...

15 September 2020 5,585 5 View

New transformer cores are designed to reduce mostly the eddy current loss. Can the ferroresonance be taken into the design account?

I mean , is it possible to manufacture a material in which the magnetic permeability is high, the saturation point is high and the demagnetization is not eazy? In this situation, the probability...

08 June 2020 3,987 4 View

How can I choose a topic in which I publish papers, since I am attracted by all electric topics, how can I specify their criteria ?

The technology is growing up very fast, and the innovations are amazing, unlimited topics are present, how to do a good job if we don't focus on only one field, or one topic.

21 May 2020 5,632 7 View

How can i run Gibson construct on agarose gel to see either assembly happens or not? If Some one can share the whole protocol or gel picture?

The size of my vector is 3.8kb and insert is 1.2kb. I have used 70ng of vector and 130ng of insert and mixed with Gibson master mix kept them for 20 minutes at 50C. I want to know is it possible...

19 August 2018 4,518 3 View

Does anyone know about using IMS bench SIPp with OpenIMSCOre ?

Hello guys Have anyone of you already used IMS bench SIPp with OpenIMSCOre? If so how can i test the registration phase? i mean what are the steps? There is really little support about that. Many...

18 March 2015 1,089 17 View

Can I simulate a communication based SIP protocol on Omnet++ simulator?

I would like to know if it's easily possible to simulate a communication based SIP on the Omnet++? If not, what are the possible tools to do that? For more clarification I would like to simulate a...

17 March 2015 9,563 0 View

Which simulator is suitable for communication protocol assessment of performance?

Hello everybody I want to test the performance of my own authentication protocol. To be more clear, my protocol is for IMS network. and i don't really know which tool should be used (omnet++ ,...

16 March 2015 4,112 6 View

Can we store blood samplesfor one day in normal EDTA vials for micro-RNA isolation?

micro-RNA

01 January 1970 1,751 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

How to estimate/know plant virus concentration to make a standard curve in ELISA after serial dilution?

Hello, I am trying to know tomato virus titer/concentration in samples and I will perform serial dilution of the positive control. I wanted to ask How to estimate/know plant virus concentration to...

13 February 2021 6,534 4 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

What is a good software for non-linear curve fitting rather than Excel and Lab Fit?

I am looking for good software for curve fitting. My curve equation is non-linear. I used Lab Fit and Excel software but could not find the curve equation with good accuracy. What software do you...

20 January 2021 5,554 13 View

Does anyone grow Mycobacteria in TECAN Spark machine to get growth curves? What setting is necessary? Any tips (use Tween20 or tyloxapol?)?

I would like to grow Mycobact. Smegmatis in 24well plate in TECAN Spark machine. So far, I was using 100ml erlenmeyer flasks with 20 ml bacterial culture w/ shaking 180 rpm in 37C . Tecan would...

10 January 2021 6,624 2 View

How does the dilution of matrix-match calibrators impact the ionization in LC-MS/MS?

Greetings. I would like to get opinions from the experts for the improvement and optimization of multiple drug confirmation by LC-MS/MS. 1. One of our method calibration curves follows the...

10 January 2021 9,559 2 View

Do polymers with different chain lengths have UV-Vis absorption peaks at different wavelengths?

I have a sample of mixtures containing polystyrene microspheres (PS-MS) with 3 different diameter sizes (bought from thermo), they all contain a green fluorescent dye inside the polymer matrix /...

06 January 2021 2,218 5 View

Why train curves are being repeated in this five fold cross validation?

Hello, I am using ...... optimizer = RMSprop(lr=1e-4, decay=1e-6) N_FOLDS = 5 EPOCHS = 20 INIT_LR = 1e-4 Train_Batch_Size = 32 Valid_Batch_Size = 32 decay_rate = 0.95 decay_step =...

30 December 2020 2,519 3 View