How to model the causal effect of working part-time compared to full-time on health?

Hi there, I have to estimate the causal effect of part-time work compared to full-time work on health. I have an unbalanced panel data set with t=2008-2017, except for year 2009. The main issue...

11 December 2020 3,170 3 View

How to correlate bacteria community data with volatile profiles and with fly community data using ordination techniques?

Carrion Ecology: What is the best way the statistically analyse the correlation of bacteria community data to volatile profiles and to fly community data across 3 time points using ordination...

24 November 2020 5,831 3 View

Does anyone have any advice for Intracellular Staining of methyltransferase for flow cytometry?

Hi All, I have been attempting intracellular staining of a methyltransferase and have been running into issues with consistency. I have attempted sequential staining with a PE secondary and have...

20 November 2020 2,314 1 View

How many casual deaths constitute scientific evidence? Do we know the risks of repeating influenza vaccination?

Italy (2014). Around 35 death after and very closer to the flu vaccination Spain (2020). At least 45 death reported in correlation with adyuvate flu vaccination and 6 time risk to died with...

22 October 2020 2,746 2 View

Use of FcR blocking reagent for B cell surface IgG staining in flow cytometry?

I would like to measure cell surface IgG in human culture of B cells. I have culture of human peripheral blood mononuclear cells (PBMCs) that were immortalized with EBV (Epstein-Barr Virus) and...

30 August 2020 6,805 2 View

After incubating overnight in blocking buffer (BSA) at 4C, how long can i incubate in Primary antibody the following day?

Hello Everyone, I usually block my membrane in BSA blocking buffer for 1 hour before incubating in primary overnight. This time i was pressed for time and decided to block at 4C over night....

05 August 2020 7,007 4 View

Request for suggestion on bimonthly journals in industrial water pollution management ?

Can anyone suggest some bimonthly journals which are either Scopus or UGC indexed in the area industrial water pollution management? thank you?

24 July 2020 2,036 2 View

Supplier for NiTi powder?

28 June 2020 6,530 4 View

Which is the most recent phylyogenetic tree for rodents?

Dear all Does someone have a recent rodent phylogenetic tree (preferably in Nexus format or any other format usable in R) or knows a publication with a recent tree? Currently I am still using...

24 May 2020 3,620 4 View

Does anyone know a school offering a BioInformatics short course in Dallas?

I am finding it pretty difficult doing an online program. Does anyone know of any school offering a short program or continuing education in Bioinformatics?

27 February 2020 6,065 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

How to obtain free lipids starting from concentration over the CMC?

I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...

17 February 2021 1,676 3 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

Remove excess hydrazine hydrate?

How can I get rid of excess hydrazine hydrate 99%?? The reaction is converting an ester into hydrazide , required excess amount of HH to be done with ethanol as solvent. I removed ethanol by...

30 January 2021 7,753 4 View

What could be possible reason of higher Drug Concentration of Liposomal formulation stability samples over time?

Hello Everyone! I have prepared liposomal formulation using Rotary Evaporator method followed by dialyzed the liposomal Suspension in Phosphate buffer to remove the un-encapsulated drug. After...

25 January 2021 7,752 4 View

Which kind of GPC solvent can be used to dissolve both lipid and salt?

I want to find a GPC available solvent to dissolve both lipid (DPPC, 2 mM) and salt (NaCl, 100 mM).

14 January 2021 3,805 2 View

How to remove ORO (Oil red O) background precipitates?

Hi ORO experts, I'm struggling with ORO background precipitates in arterial tissues. The protocol I use works very well on human atherosclerotic plaques which are full of lipids and I don't...

13 January 2021 7,110 1 View

Why the lipid accumulation levels in my 3T3L1 does not match with my adipogenic and lipogenic gene expression levels?

I am using the 3T3-L1 cell line to discover the adipogenic effects of a natural compound and its derivatives. I conducted a lipid accumulation assay using fluorescent Nile Red dye (Adipored) for...

10 January 2021 4,360 1 View