I'm preparing liposomes by thin film evaporation method. I'm wondering how many water I sholud add during hydration step?
The next step will be the extrusion using 100nm PC membrane.
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hello dear researchers, I would like to know about available in silico tests that could predict the activity of ligands (small molecules) against the target protein. I have performed docking...
08 July 2024 4,724 4 View
Still alive but new info from last hospitalization a week ago. Per Endo ... Covid remapped the electrolyte panel. A high potassium 5.5 actually is low and the patient needs K+ Blood glucose at...
21 May 2024 4,780 0 View
The past few months I have started testing the selective androgen receptor modulator (SARM) GSK2881078 to study its ability to increase muscle growth and/or counteract muscle atrophy. I have done...
20 May 2024 8,747 0 View
Can hydrogen peroxide stimulate magrophages for the induction of nitric oxide? What is the protocol?
16 April 2024 1,409 1 View
For validation by qPCR of several microRNAs, is it necessary to measure normalization miRNAs in each plate in addition to the miRNAs under study? Or can you make plates of microRNAs and then...
15 April 2024 6,393 1 View
Hello everybody, I've been trying to fix the FTIR attached to this enquiry but I havent been lucky at all. I would like to know if anyone would be so kind as to help me fix this spectrum or tell...
25 March 2024 7,648 0 View
I need a little help, how do you make "Traits" in PopArt? I need to make a network of haplotypes on a map
14 March 2024 2,316 0 View
Hello, I wanted to ask for help with protein modeling programs (not servers) with a free commercial license. thank you
13 March 2024 6,173 0 View
I would to delete up to 33 bp from a plasmid to generate deletion mutation. Has anyone tried using Agilent Quikchange lightning site direct mutagenesis to delete such a long region? How feasible...
06 March 2024 8,136 5 View
Hello, I'm looking to dissolve soy lecithin in order to create a liposome. I've tried using various solvents like pure ethanol, methanol, and a mixture of ethanol and methanol but they didn't...
02 August 2024 6,980 4 View
I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
02 August 2024 3,143 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
I have an issue with SEM imaging of liposomes. When measured by DLS, they appear to be around 120 nm, but when using SEM with simple air drying, they appear much larger, around one micrometer.
25 July 2024 3,395 3 View
I am looking for a lipid waste disposal method, keeping in mind the environmental, health and safety aspect of lipid waste. Could someone please provide guidelines or the lowest acceptable...
25 July 2024 637 5 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 5 mg/mL?
18 July 2024 9,831 0 View
Hi All I am looking to get your opinions, I have perfomed liposome mediated DNA transfer into bacteria, the DNA is a plasmid with msfGFP and when I induce the plasmid I see the fluorescence, and...
16 July 2024 4,698 4 View
Hello everyone, I am trying to encapsulate an essential oil in the liposomes using the heating method and encountering some issues. I am following the protocol described below, but my solution...
10 July 2024 4,083 3 View
I am currently trying to measure the entrapment efficiency of curcumin in liposomes. Typically, this measurement can be done by centrifugation, and I have followed the protocol outlined by Asif et...
01 July 2024 7,007 1 View