For gene expression study how many days we can store the organ sample. If suppose we store more than 3 month is there any changes on organs.
Storing the sample in RNAlater reagent would stabilise the RNA, hence can be stored for a longer period. Following are the appropriate resources for your question:
1. Bahn S, Augood SJ, Ryan M, Standaert DG, Starkey M, et al. (2001) Gene expression profiling in the post-mortem human brain--no cause for dismay. J Chem Neuroanat 22: 79-94.
2. Soutourina O, Cheval L, Doucet A (2005) Global analysis of gene expression in mammalian kidney. Pflugers Arch 450: 13-25.
3. Cookson W, Liang L, Abecasis G, Moffatt M, Lathrop M (2009) Mapping complex disease traits with global gene expression. Nat Rev Genet 10: 184-194.
4. Consortium GT (2013) The Genotype-Tissue Expression (GTEx) project. Nat Genet 45: 580-585.
5. Goh K-I, Cusick ME, Valle D, Childs B, Vidal M, et al. (2007) The human disease network. Proceedings of the National Academy of Sciences 104: 8685-8690.
Hope this help!
That would depend on the reagents or chemicals in which your tissues are stored. For instance with RNAlater your can store tissues for months to years at -20 or -80 oC without compromising the RNA integrity. With the same chemical preservative you can store at 4oC for about one month or at room temperature for about one week.
All the above answers are great! I would also highly suggest the use of the new Paxgene Tissue tubes! Designed specifically for tissue.
http://www.preanalytix.com/products/tissue
RNAlater is highly recommended for soft tissues such as liver, kidney, small intestine e.t.c But if you work with material such as bone or cartilage, in which therefore the RNAlater cannot penetrate the tissue, it would be best to keep them at -80.
I have had tissues and RNA stored at -80 for up to 2 years and the RNA is still absolutely fine.
If you freeze the tissues at -80ºC fast enough right after extraction the RNA will be fine for months/years
RNA is completely useless and broken down in 15 minutes of fresh extracted tissue. Different RNAs all have different rates of degradation and there is no way to be sure your RNAs of interest or their controls will be similar. Sure, perhaps you could extract tissue quickly enough and freeze it, but you still need to thaw it at some point, and your RNA will be directly affected.
Don't leave your samples to whim. Stabilization reagents are cheap when considering the benefit they provide.
you can use reagents like RNAlater or you can work with liquid nitrogen, both work fine if you use them properly.
good luck!
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