Adam Clark

6 Questions 6 Answers 0 Followers

Questions related from Adam Clark

Papers that accept discovery studies?

Hello colleagues, I was wondering if I could ask for general advice or guidance on something? I tried to submit a paper recently, but it got rejected on this occasion. Essentially, we did a...

11 November 2019 1,690 2 View

Higher Bands in a qPCR gel run?

Greetings everyone, I was wondering if I could run something by you all? I have been running gels to confirm PCR product size of primers. I have 6 primer pairs to design for 6 genes, and so far I...

03 March 2017 9,593 4 View

Identifying gene promoter sequences for methylation study?

Hi everyone, was wondering if I could run something by you all? I need to identify promoter sequences in several genes of interest as we are interested in doing bisulfite sequencing to determine...

02 February 2017 4,591 4 View

How to reverse flush an HPLC column which has had ammonium acetate flown through?

Hi everyone, I was wondering if I could obtain some advice with something? We've recently developed an HPLC method in which the mobile phase has a small amount of ammonium acetate dissolved in...

08 August 2016 8,376 7 View

Best calibrators for qPCR when analysing disease?

Hi everyone, I was wondering if I could please receive some feedback with something? I am currently investigating what genes are being up regulated/down regulated in pigs suffering from a...

05 May 2016 855 11 View

Is there a method for detecting very low transcripts for qRT-PCR?

Hi Everyone, I was wondering if I could please ask for help with something? I'm currently attempting to analyse expression of CYP26A1 in cartilage samples through qPCR. I have ran my samples...

01 January 2016 7,131 5 View