How many cells (parasite Plasmodium falciparum cell pellet) need to lyse per mL of lysis buffer?

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Boopathi Subramani

You can use 10 ml lysis buffer per gram wet weight of cell pellet.

Hiasindh Ashmi

Thanks Boopathi for your reply.

I have used 10 ml lysis buffer per gram wet weight of pellet for cloning and expression of protein in E. coli cells. Since my study now is Plasmodium falciparum culture, I was having doubt whether it will be the same or different.