Hiasindh Ashmi

9 Questions 23 Answers 0 Followers

Questions related from Hiasindh Ashmi

Can anyone help me how to remove non-specific band in PCR? I have tried gradient PCR, increasing the MgCl2 concentration. What can be done more?

Hi, I have designed a primer pair for amplification of malaria parasite PfCRT gene. When i did gradient PCR with 1.5 mM MgCL2 conc, I got my desired band at 700 bp, along with a non-specific band...

04 April 2018 5,483 37 View

How can I store RNA sample for plasmodium falciparum at -80°C, either in TRIzol or RNA later? Which is suitable for transport?

Hi, I'm working with RNA extraction from Plasmodium falciparum samples. I want to send the culture sample for my friend. Is it best to store the sample at TRIzol or RNA later and also for...

03 March 2015 4,059 4 View

How many cells (parasite Plasmodium falciparum cell pellet) need to lyse per mL of lysis buffer?

I am trying to do cell lysis of Plasmodium falciparum pellet for 2D gel electrophoresis. Could anyone tell me how many/ much cells (either based on OD 595, or weight of cell pellet) needed to lyse...

04 April 2014 3,896 2 View

What is an appropriate gel system/method to study the oligomeric state of peptide ~3kDa?

I am trying to study the oligomeric state of peptide of size ~3kDa with theoretical pI of 10.19. I have tried using 15% SDS-Glycine system and done silver staining, but no bands visible. Then, I...

12 December 2013 9,421 4 View

What percentage of purity of peptide/ protein is required for fluorescence quenching assay?

I want to perform a fluorescence quenching assay of peptide, to confirm if it is a transmembrane domain. The peptide has one tryptophan in the middle of peptide (28 residues). Am planning to do...

11 November 2013 8,618 6 View

Could anyone help me with native PAGE for basic proteins?

I am trying to run native page electrophoresis for basic protein of pI 10.43, with normal sds page gel, removing all the sds and run the sample without denaturation. The protein is 3.4 kDa and i...

10 October 2013 5,050 11 View

How to calculate Stern-Volmer constant from fluorescence quenching studies?

I am trying to do phenylalanine quenching studies for my peptide. It has 2 tyrosine and 2 phenylalanine and no tryptophan in its sequence. Its a transmembrane domain, so I want to find out whether...

10 October 2013 5,094 16 View

Tools for molecular dynamics stimulation?

I am working with a membrane protein and crystallographic structure for the protein is not available yet. And am trying to predict the structure using in-silico methods and have predicted using...

08 August 2013 5,551 4 View

How to predict structure of protein with identity less than 40 and its a homodimer protein?

I want to predict the structure of my protein of interest, with 424 aminoacids of unknown structure. When i blast my protein against PDB database, only one match came with identity less than 40 %....

07 July 2013 1,160 7 View