Hi all,
I want to know how long the fluorescent signals of Fos- or Arc-TRAPed neurons lasts.
TRAP (Targeted Recombination in Active Populations) reference papers shows that the animals were sacrificed one week after injecting tamoxifen or 4-hydroxytamoxifen and behavioral induction. However, I think it is permanent and the fluorescent signals of Fos- or Arc-TRAPed neurons wll be visible after a week. Is it fine to detect FosTRAPed neurons 3 or 4 weeks after behavioral induction?
I am looking forward to hearing from you.
Thanks,
Tae-Yong
Hi Tae-Yong Choi,
With TRAP and TRAP2 mice the expression of the TdTomato will be indefinite after Cre recombination since it is driven by the CAG promoter if I remember correctly. You could image months after tamoxifen exposure and you will still have florescence. It is advisable to include controls without tamoxifen so that you can see the percentage recombination as that could potentially be higher the longer you wait.
Hope this helps,
Ezequiel
Thank you nery much for your advice! I will check it with controls without tamoxifen that you mentioned.