I started with high density say 1 million cells per 6 well, I was able see spheres with in two hours and in 48 hrs could see some spheres. Some spheres remain attached should I continue the induction protocol for the attached sphere?. Wat do I do after seeing floating sphere plate directly for neuronal diffferentiation in PLL/FN double coated plates and just maintain them in DMEM F12 media with 2% FBS , by changing media every 2 days up to 10 days or should i supplement them with B-27, bfgf and EGF