As far as i know ascorbate peroxidase is very labile.. You should measure it immediately.. You keep them at 4°C or freeze them ? There is a paper where they tested freeze thaw cycles..
As per my openion, each time its good to prepare fresh solution for any kind of test. And i also suggest you to keep your extract in desicator, so that you can avoid oxidation and also you can remove some moisture from the extract too...
good luck
Obviously, storage of the enzyme extract at 4 ºC for 20 days is a very bad idea.
Dear Prabakaran,
Majority of enzymes extracted in buffer will lose their activity, when stored at 4oC for over 12 to 24 h. If you want to store for longer time you can freeze and store them in freezer (may be at 20oC). However, please ensure that the samples are not subjected to repeated freezing & thawing (this is often experienced due to power failure, which many of us might overlook). Whenever you wish to measure enzyme activity, you can take out the sample, thaw it & measure activity(activities) of whatever enzyme(s) you are interested in. Please do not refreeze or store the sample(s), ones you thaw it(them).
Hi. I am agree with Pardha and Zhang. The plant organs and enzyme extract shoul be store at -20 or -80 C. For long time the best option is freezing and storing in -80 C.
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