Norbert Bittner

4 Questions 73 Answers 0 Followers

Questions related from Norbert Bittner

Can an increased level of hydrogen peroxide reverse the reaction of superoxide dismutase in an assay ?

I'm having the problem that in my SOD assay (NBT assay, Pyrogallol assay) the sample reaction is showing a faster O2- production than the blank of the SOD standards. Means that the samples are way...

29 June 2015 5,841 11 View

Is it possible that a ligand of a protein stays bound to the protein after Native PAGE and subsequent electroelution ? Any references maybe ?

I have an insect protein mix and separate it with a modified BN-PAGE protocol. The protein of interest is electroeluted and then further used for a bioassay. Every step is at a constant pH of 7.0...

05 December 2014 1,527 6 View

Is the Commasie used in BN-PAGE interfering with protein activity after gel elution of cut out bands?

Do I have to destain before eluting out my protein of interest to keep it active?

10 September 2013 10,131 2 View

Anyone have experience with Borax Agarose gel electrophoresis for RNA ?

I use them for DNA gels regularly. Any precautions for RNA?

21 August 2013 7,198 1 View