The extracted protein from a plant sample was immediately dried and stored in deep freezing condition at -80°C. How long does this remain stable until estimation and analysis?
It can be kept for long years without change
Bfgf was aliquoted in media and transported at 22 degee Celsius. How long it is stable
15 June 2024 3,010 2 View
We were imaging platelets under SEM. We got this structure. Any recommendation on what this structure could be will be of great help. Thank you
02 May 2024 3,320 4 View
Looking for biochemical assays for quantitative estimation of Albumin and Casein protein.
28 April 2024 4,285 3 View
I tested gene expression by RT PCR followed by Western blotting to test protein expression. I get an inverse correlation with up-regulation at mRNA level and down-regulation at the protein level....
05 April 2024 4,679 6 View
I have been getting these long smeared lanes for very long in western blot. Every time I make fresh buffers and even I have changed the antibody brand. Nothing helped. Please provide suggestions...
05 November 2023 6,888 4 View
I used Spe1 and BamH1 restriction enzymes for checking presence of plasmid in EP1 300 strain. It had a 40kb insert size. I have got faint bands at 6kbp and at 2kbp. But the first band is smeared....
03 August 2023 7,901 0 View
After analyzing PI data of LN229 cells (glioma cell line), I observed that even the control (untreated) population had the following cell cycle proportion: G0/G1 : 79% S : 8% G2/M: 13% Is this a...
11 July 2023 6,642 2 View
When i started my real time pcr experiment i was using applied biosystems power up sybr green qpcr master mix. i was getting my results with a proper melt curve of my target gene at 83.5 degree...
21 May 2023 7,007 1 View
It isnt dissolving in any solvent, i have tried water, methanol, DMSO and ethanol and also tried changing the temperature and pH. Can anyone suggest a suitable solvent or a methodology?
15 February 2023 3,689 5 View
I need maximum concentration of chitosan to maintain the positive zeta potential of calcium nanoparticles. Please add your views....
30 January 2023 8,813 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?
03 August 2024 7,829 0 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View