I already did PCR and purified my PCR products from gel. Now I am trying to do a clone in a vector. The problem I'm facing though is wondering how long a polyA tail remains in a PCR product.
Why do you have a polyA tail in your PCR product and why do you assume that it might get lost over time?
If you used Taq polymerase, you end up with a single A at the 3'-end, while most proof reading polymerases produce blunt ends. And if you have an overhanf, it is usually there to stay, unless you deliberately blunt the product.
Hi Avjit
Do as sugegsted by Christian above...or use a 'T-tailed' vector for cloning such PCR amplified fragments
bye
As it is a DNA fragment, I don't think it will be having Poly A Capping. The function of poly A capping in an RNA is just to provide stability. As your's is DNA fragment, don't worry about the Poly A tail.
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