Avijit Das

4 Questions 10 Answers 0 Followers

Questions related from Avijit Das

How can I remove N-terminal His tag from pET28a express protein ?

I have a clone of 9kDa protein in pET28a, at EcoRI and HindIII. Can anyone suggest me, how can I remove the His tag?

06 June 2018 4,533 8 View

How long does a polyA tail remain after PCR?

I already did PCR and purified my PCR products from gel. Now I am trying to do a clone in a vector. The problem I'm facing though is wondering how long a polyA tail remains in a PCR product.

20 March 2014 3,985 3 View

Can anyone advise why do we use PEG-8000 for phage precipitation along with NaCl?

Why PEG-8000? Why NaCl?

30 January 2014 1,180 2 View

What to do, when research proposal get stolen and copied ?

What to do, when my proposed research proposal stolen and copied, and get funded by DST SERB CRG scheme. Will share the all detail details upon request,

01 January 1970 3,394 11 View