How long does a BLAST with the WGS database on NCBI takes?

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Como ficarão as vagas de emprego com o advento da Inteligência Artificial?

Em relação aos empregos que estão sendo substituídos pela inteligência artificial, como está sendo organizada a formação e a realocação dos colaboradores? Pois muitos empregos ficarão escassos, e...

31 July 2024 6,864 1 View

My question concerns MTT cell viability assay?

Despite not having cells in the media, I am getting purple color. I have tried many troubleshooting methods, varying media types, and even different MTTs from different companies to figure out the...

21 July 2024 9,914 1 View

Why is linearity somewhat lost when plotting the Stern-Volmer (SV) graph?

When preparing a graph of fluorophore intensity vs. analyte concentration, the linearity shows an R² of 0.999. However, when plotting the same data as Fo/F vs. analyte concentration, the linearity...

13 July 2024 7,131 0 View

I need help to write a good simulation of the solar system and its travel around the galaxy.Can you help me finish the code?

This is the code I have. I would need: An initial configuration (position and momentum), the orientation of the ecliptic with respect to the direction of travel by the sun, The orientation of the...

09 July 2024 6,063 2 View

What do you think is the main advantage of cell membrane-camouflaged nanoparticles, according to your experience?

04 July 2024 6,902 2 View

A variable moderating multiple relation - how can I test hypotheses using amos?

How can I analyse the relationship between five independent variables and one dependent variable, with a moderating variable influencing each of these five relationships? How can I test hypotheses...

03 June 2024 6,221 1 View

How to obtain nanoparticles from microparticles?

If you prepare drug-loaded nanoparticles but only obtain microparticles (~2,000-4,000 nm), PDI~0.2, how can you reduce the size to reach ~100 nm?

02 June 2024 2,693 4 View

How to study the interaction established between two drug molecules?

How to know if two drug molecules interact with each other, the strength (and type) of interaction and the possibility to form nanoparticles?

02 June 2024 4,304 1 View

Any experience in fibroblast centrifugation?

We are experiencing different problems pelleting fibroblast: a) From the resuspension of the comercial vials (after defrosting, we resuspended in 5ml complete DMEM + L-Glutamin + FBS +...

28 May 2024 3,529 1 View

Fibroblast trypsinization in Hanks medium with Ca and Mg?

We are setting up fibroblast cultures in a new location and we had to change the mediums suppliers. At the trypsinization step trypsin did not work and we realized our hanks medium has Ca and Mg....

28 May 2024 6,052 2 View

Why my negative control siRNA is decreasing the target gene's expression?

Hi Everyone, I'm using an siRNA kit to knock down a target gene. The kit guarantees that the negative control doesn't target any sequence in mouse genome, and when I use BLAST I don't find any...

23 July 2024 2,673 6 View

How to use NCBI datasets ?

I have been trying to extract genome from NCBI using their dataset tool, however some examples seem not to work : ./datasets download genome taxon "Homo Sapiens" --annotated --assembly-level...

20 July 2024 1,339 2 View

How to download fasta files in "gene features" format from NCBI with multiple samples in R?

Hello, I was attempting to download some sequences data from NCBI. The format "gene features" could help me extract certain gene fractions using the names, but I found that it seems to be...

04 July 2024 9,875 1 View

Problems to find a region of a Virus gene?

We have a couple of primers but when we make a blast for those primers we see two results: When I use Blast from NCBI, I found many lineages which anniling with many lineages. But when I want to...

01 July 2024 7,644 3 View

Query Regarding tRNA Annotation Order in Feature Table for NCBI Submission?

Dear Sir, I hope this message finds you well and in good health. I am writing to seek your guidance regarding an issue I encountered during the annotation of my phage sequence using DNA Master....

30 June 2024 8,650 0 View

Is there any bioinformatic tool that can download batch DNA seq and translate them into multiple aa seqs?

Hi, everyone I would like to know if there is there any bioinformatic tool that can download batch DNA seq using imported NCBI accession numbers (upto 100 sequences) and translate them into...

19 June 2024 1,917 2 View

How to design primers and run PCR in SerialCloner?

I am working on a paper for my bioinformatics class, where we are running an analysis of transcriptomics on A. thaliana col-0 about the difference in gene expression in the temperatures of 21 and...

12 June 2024 8,822 1 View

How to upload 16S and metagenomic data from fecal samples to NCBI SRA database?

Hi there, I have some 16S and metagenomic sequencing data from mice fecals and I want to upload them to NCBI Sequence Read Archive (SRA). I don't know what NCBI package is suitable for my animal...

03 June 2024 9,451 3 View

BLAST - missing results?

Hello, in January, I successfully used BLAST to find a homologue of HDV in a TSA dataset from a lizard. However, when I recently attempted to search for that sequence again, I couldn't find it in...

02 June 2024 7,415 1 View

How to use the BLAST primer to check the possible bonds of primer?

Hi everyone, I run PCR to checked presence of specific gene in Clostridium perfringens but I received 2 bands, one band showed product size expected and another one showed higher product size. I...

31 May 2024 645 1 View