Hello Guys,
My lab usually leaves cell to attach on cell plate overnight after cell subculture, but we don't know how long it usually take to attach on the plate. Do you know how long? Could it be a few hours? Sorry, I couldn't find through my google search.
Cheerio,
Dear Asa,
It will depend from line to line, but for example with my breast cancer cells after 6 hours they are completely attached.
Matteo
Depends on type of cells and state of the cells as well as culture conditions and coating. Are they senescent/frozen/treated prior to the seeding? Cells start adhereing from minutes (healthy astrocytes mechanically passaged onto laminin) - 6 hours (fibroblasts on gelatin) - overnight (neurons on properly coated vessle)
Staffan has provided a good answer. It also depends on whether youi subculture from an already revived plate or freshly from liquid N2. If you trypsinized the adherent cells then replate them probably only need a few minutes for some of them to attach. For both endothelial and sommoth muscle cells I have seen them attached withing 20 minutes whereas from liquid N2 it may take 2-4 hours.
Morning folks.
I'm growing Rin-5F cells (pancreatic) lines from frozen vial and its now two weeks not attaching. Please help me. this is now frustrating so much.
cheers
I agree, it depends on the cells type. I have cultured neural stem cell line, and lung and skin fibroblasts and they attach in 2-3 hours. But My MCF-7 cell line is not attaching even after 4hours.
It really depends on cell type. Can go from minutes to several hours.
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