26 December 2016 1 4K Report

we use FBS and 10% DMSO as cryo media for preservation of mammalian cells . how long it can be stored or used and is their any special condition for storage? please suggest.. 

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Is there any protein-protein docking software available to dock multiple proteins at a time?

I want to dock a secretory protein having about 110 amino acid residues with more than thousand receptor proteins to find the best interaction. Is there any highthroughput technique available to...

06 January 2023 1,420 2 View

Do 1-day protocols for miRNA in situ hybridization work for FFPE tissue sections?

The conventional protocols for miRNA hybridization for FFPE tissue sections stretch for 2-3 days, with prehybridization and an overnight hybridization steps. But some companies like thermo and...

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What is the procedure for antioxidant activity by H2O2 and NO?

Step by step procedure

10 January 2022 5,740 3 View

How much is the role of education in living a happy life?

What is the purpose of education and what we get from education?

23 September 2020 6,097 4 View

How to select a water model and force field for a molecular dynamics study for protein? How to decide the length of simulation?

I am working on a kinase-inhibitor complex. I wanted to perform a molecular dynamics simulation for the same. While going through the literature, I came across several options for the water model...

04 July 2020 8,106 0 View

Which RMSD value from Chimera should be considered while selecting the homology models developed using different templates?

I have developed a homology model with different templates. For checking the overall deviation from template structure, RMSD calculation was performed using Match Maker option in Chimera. It gives...

29 June 2020 7,133 0 View

What should be the standard glucose concentration to be used in high glucose studies, that match the physiological levels?

Forgive me for being Naive. I have read that the postprandial concentration normally doesn't go above 140mg/dl, anything above it is considered diabetic. for instance:...

23 July 2019 2,088 4 View

What could be the possible reasons behind the cells dying in the culture plate in few wells while surviving in other, media conditions being same?

I work on MC3T3-E1 cell lines. The cells appear absolutely fine in flasks, but when I seed them in 12/24 well plates for mineralization (nunc thermofischer plates), then cells appear to die in few...

03 February 2019 1,772 3 View

At what stage of the cell cycle do cells arrest during subculturing process? Does the culture become synchronous after few passages?

During animal cell culture, confluent cells are trypsinised, centrifuged, resuspended and finally subcultured. I read somewhere that contact inhibition is used to make the cultures synchronous....

03 February 2019 561 3 View

How to extract particular section from text data using NLP in Python?

I have lot of CV (text documents). From these data I want to extract particular section of 'Education Qualification', 'Experience', etc.

03 January 2019 4,590 12 View

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