GAPDH, Beta actin
@Rahaman,
What do you mean by opening a gene!!!!!
I can't get you....
Did you mean primers for those housekeeping genes or antibodies against those proteins?
Publication in both groups of journals are should not be used for academic promotion.
11 December 2019 5,531 8 View
I am looking for a tissue lysis buffer for immunophenotyping. TQ
11 December 2018 8,240 0 View
I have run MTT for crude extract, pure compound and essential oil at the same time. I have used same batch of the cell, same number (0.8X 10,0000 cells/ml), same technique and at the same day, but...
06 July 2018 5,503 2 View
Some of my submitted manuscripts (5) got rejected by the journals, and want to submit into another journal, but I do not have enough time to format. If any one can provide me with the soft ware...
31 December 2017 3,055 36 View
In recent years several new mouse models for lung cancer have been described. These include models for both non-small-cell lung cancer (NSCLC) and small-cell lung cancer (SCLC). Tumorigenesis in...
11 December 2015 8,196 4 View
But like other treatments, it often causes side effects. These are different for each person. They depend on the type of cancer, location, drugs and dose, and your general health. How long the...
10 November 2015 7,906 3 View
Polymerase Chain Reaction is widely held as one of the most important inventions of the 20th century in molecular biology. Small amounts of the genetic material can now be amplified to be able to...
11 December 2014 6,526 47 View
I already used EndNote during my PhD study, but now want to learn more soft wares. I will be appreciable if any one help me in this issue. TQ
10 November 2014 5,656 10 View
Can anyone guide and help me to promote the growth of human melanoma cell line (SK-MEL-2) and murine melanoma cell line (B16). We bought both of them directly from ATCC 2 months ago. At begining...
06 July 2014 3,241 12 View
When we want to screen or test the effect of a natural product in cancer cells, we have to know how safe is it. Why do some articles mention antiproliferative and others cytotoxicity assays for...
05 June 2014 3,280 20 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Do experiments on behalf of Our Business: Laboratory animal rearing Animal modeling Experimental animal behavior detection, image ultrasonic detection, electrophysiology platform Organoid...
05 August 2024 5,829 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I am in search of a modified Hummer's method, which can be used to synthesis graphene oxide within 6-8 hours. As I am a student it is not allowed for us to work after 5 in the laboratory. So I am...
01 August 2024 8,368 2 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View