I know cancer cells can be stored in this way for long term storage. How about normal cells like NHEKa? DMSO will be used as the cryoprotectant.
Hello Phylia Chew
I doubt whether cells will remain viable for long term preservation at -80 degree C. You have to store the cells in liquid nitrogen for long term storage. Cells (including cancer cell lines) may be kept at -80 degree C for a maximum of 1-2 years after which the cell viability may decrease, although there are claims that human and other mammalian cells can be stored at −80 °C for long term (8 years). Please refer to the paper attached below.
Article Long-term Cryopreservation of Human and other Mammalian Cell...
I am also attaching a link below introducing a serum-free cell freezing medium named BAMBANKER that claim for long-term storage of cells (tumor cells, stem cells and normal cells) at -80°C . You may try this product.
https://www.nippongenetics.eu/app/uploads/2017/01/BB0_Bambanker_Manual.pdf
Best.
Malcolm Nobre Thanks for the answer, sir.
I'm wondering if I just need to temporarily store and use them within 2-3 months can -80 degree Celsius hold their viability long enough? We don't have a liquid nitrogen tank at the moment to store cells for long-term storage, so whatever new commercially purchased cells coming in will be cultured immediately to be used until the cells die off by themselves, which I find is a waste of resource and the work has to be continued on with no rests in between. I'm not sure if it is worth trying to do so or if this will definitely not work for normal cells.
Phylia Chew temporarily you may store the normal cells for 6 months using DMSO as the cryoprotectant. Do not store beyond 8 months. You should make a stock of every commercially purchased cell line consisting of a few vials in -80 degree C which will be helpful in case anything goes wrong with your working culture. You may thaw the frozen cells and continue working without any breaks. It saves time and resources.
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