GFP antibody for flow cytometry?

Hello, I have been trying to detect the YFP expression in my YFP-tagged mouse model with anti-GFP antibody for flow cytometry. My question is, is this normal to observe a shift in GFP expression,...

23 July 2020 4,300 12 View

Difference between FSL FUGUE and TOPUP?

06 September 2017 1,360 9 View

Is there any method to isolate rust, smut and mildew fungi on fruits and culture them in agar plate?

27 August 2017 2,458 3 View

How do we create omniphobic surfaces through coating or grafting on hydrophobic hollow fibre membranes?

Are there any novel chemicals that are worth trying?

07 February 2017 347 1 View

Does anyone know how can I investigate whether a compound is inhibiting a bacteria cell via pro oxidant effect? Any paper to recommend?

I have a molecule which showed inhibition activity to bacteria. I suspect it could be via pro oxidant effect (generation of free radicals). How can I investigate on this. Appreciate if someone can...

06 October 2016 8,217 3 View

How to calculate whether the spiked amount of metal ions equal to the total amount in a close system contained liquid and solid phase?

For an example, 1mg/L of copper ions was spiked in to a system containing water and soil. After few day, the concentration of copper ions in water and in sediment were determined after acid...

13 September 2016 7,011 6 View

How to control the nano-size of particles by using sol-gel method?

I have prepared a nanosilica by sol gel method, however, the size distribution is very wide. Does anyone know how to narrow the size distribution of the nanosilica?

11 October 2012 3,418 37 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

can I treat waste water containing solvents and alcohol with electrocoagulation?

there are solvents and alcohol in the waste water of a factory that produces disinfectants. Increasing this waste water requires very serious operations. Can I treat this waste water with...

27 February 2021 2,609 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View