Phylia Chew

5 Questions 4 Answers 0 Followers

Questions related from Phylia Chew

Is serum starvation necessary for cell culture?

If I am using growth medium containing BPE instead of FBS, is it still necessary for me to serum starve my cells for 24 hours before subjecting them to drug treatment? My purpose is to study cell...

15 October 2023 2,442 1 View

Does anyone have any idea on the range of concentration of Gefitinib that causes cell cytotoxicity in NHEK cells?

I'm trying to screen for a suitable range of concentration of Gefitinib for my subsequent ELISA assay, the final aim is to select a Gefitinib concentration that does not kill cells+give off...

01 August 2023 5,102 1 View

Can I expose my drug stock under UV in the biosafety cabinet?

I have prepared my drug stock with filtered-sterilized DMSO outside the biosafety cabinet, can I expose them under UV for a few minutes together with my cell culture tools before further diluting...

17 June 2023 6,795 3 View

How long can normal human epidermal keratinocyte cells (NHEKa) be stored under -80 degree celcius?

I know cancer cells can be stored in this way for long term storage. How about normal cells like NHEKa? DMSO will be used as the cryoprotectant.

09 May 2023 9,901 3 View

How do i prepare TRF stock solution for cell culture study?

Can I use 100% DMSO as the solvent to prepare tocotrienol rich fraction (TRF) stock solution? I plan to dilute it afterwards using KGM2 as I am using the TRF as a treatment for my normal human...

26 April 2023 872 2 View