How many days can I keep deparrafinized IHC glass slides in PBS without affecting my staining quality>
Dear all. I need a couple of examples that were mentioned in a paper, a working paper or a technical repot (not a news media article) of falsification of school reports on teachers working...
25 June 2024 4,040 2 View
I am doing western blotting for sample after PFA cross-linking. And, indeed, I see something which looks like cross-linked band after PFA, but it MW is ~30 kDa smaller than expected. I would like...
08 January 2024 9,586 3 View
Project management seems to be an obvious application for the queueing theory. Risks within the projects come and go. Projects within a portfolio get started and finished. Teams get occupied and...
13 August 2023 8,657 1 View
We are planning to perform pull-down assay, but I am a new in that field. Which tag for affinity purification of the “bait ” protein is better? is there really significant differences between GST...
21 June 2022 1,884 2 View
My gene of interest is lowly expressed. What is the maxium amount of template (ng) one could use for RT-qPCR to detect the gene expression? I used 10 ng with no signal at 40 cycles.
09 February 2021 8,913 7 View
I have been experiencing this problem while IHC-p staining my brain tissues. Staining is frequently uneven with gradient-like staining pattern (around 50% of my slides are affected). This happens...
29 October 2020 8,075 4 View
Are there any researches about preschool childcare in Latin America? Next year I plan to start a project about preschool childcare systems in different countries and their influence on the...
18 September 2017 6,497 3 View
Can one perform a Dunnet`s post hoc test for one-way ANOVA analysis if the distribution is NOT normal?
15 November 2015 1,727 3 View
I need a very narrow glass capillary for my research. How can I make a glass capillary with an inner diameter of around 200 to 500 nanometers?
08 August 2024 8,915 0 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
Greeting How many events should be counted for the analysis of apoptosis with nuclear staining with Hoechst 33342 whit microscopy ? Are 100 events count sufficient and three repetitions for...
25 July 2024 7,520 0 View
Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...
23 July 2024 2,844 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View