We are planning to perform pull-down assay, but I am a new in that field. Which tag for affinity purification of the “bait ” protein is better? is there really significant differences between GST and His-tag in case of pull-down assay of human samples? I have two “bait ” proteins, both bellow 100 kDa.
As I understand, His-tag is smaller, but gives higher risc of contamination with unspecific proteins.
Hey Arseniy,
Can you only decide between these two options? I can recommend the Strep-tag as an alternative to theses tags that you already mentioned. You can find information regarding pull-down assays here: https://www.iba-lifesciences.com/applications/protein-protein-interaction/#nav-ip-co-ip-pull-down
The Strep-tag is also a small tag, but in contrast to the His-tag, it has specific elution conditions and therefore you get highly pure target proteins. I attached a White paper that compares these two tags. Maybe you're interested in reading it :)
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